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About in cell western blot

Electro-transfer is almost exclusively the contemporary transfer method of choice due to its speed, uniformity of transfer, and transfer efficiency. Electro-transfer relies on the same electro-mobility principles that drive the migration of proteins during separation in PAGE. The gel, membrane, and electrodes are assembled in a sandwich so that proteins move from gel to membrane, where they are captured, in a pattern that perfectly mirrors their migration positions in the gel.

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