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About indirect elisa

ELISA is one of the most commonly used labeled immunoassay techniques. It is based on an enzyme-labeled antibody capable of detecting an antigen immobilized to a solid surface, 96-well or 384-well polystyrene plates. A substrate is added to produce either a color change or light signal correlating to the amount of the antigen which presents in the original sample. It is a simple and rapid technique to detect antibodies or antigens attached to a solid surface. Being one of the most sensitive immunoassays, ELISA offers commercial value in laboratory research, diagnostic of disease biomarkers and quality control in various industries.

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